Molecular Detection of Chilo infuscatellus
نویسندگان
چکیده
The Chilo infuscatellus (Snellen; Lepidoptera: Pyralidae) is the main pest of sugarcane in China. The shortage of easily distinguishable morphological characters especially in early stage make it challenging to diagnosed and promptly take steps for pest management. In the present study, we described a PCR method for the molecular identification of based on barcode region of COI sequences between C. infuscatellus and four other sugarcane borer species. A 285bp fragment was successfully amplified from all life stages and different geographical populations. Sensitivity tests revealed that diagnostic bands were generated as low as the DNA template concentration of 5 ng/µl. Our work demonstrated a rapid and accurate way for the molecular diagnosis of C. infuscatellus.
منابع مشابه
Silencing of Molt-Regulating Transcription Factor Gene, CiHR3, Affects Growth and Development of Sugarcane Stem Borer, Chilo infuscatellus
RNA interference (RNAi) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. Development of reliable methods for production and delivery of double-stranded RNA (dsRNA) is the major challenge for efficient pest control. In this study, Chilo infuscatellus Snellen (Crambidae: Lepidoptera) was fed with CiHR3 dsRNA expressed in bact...
متن کاملECOLOGICAL STUDY OF THE SUGARCANE MOTH BORER, Chilo tumidicostalis (HAMPSON) (LEPIDOPTERA: PYRALIDAE) AND ITS NATURAL ENEMIES
Sugarcane, Saccharum officinarum L., is one of the most economically important crop of Thailand. It is the main source of sucrose, and is used in sugar and alcohol production. Insect pest complex is the most important limiting factor in sugarcane production. Among the sugarcane insect pest complexes, the sugarcane moth borers complex is considered most important. Chilo infuscatellus Snellen, Ch...
متن کاملPhotoremovable protecting groups as controlled-release device for sex pheromone.
Photoremovable protecting groups (PRPGs) were demonstrated as a delivery device for controlled release of pheromone under both UV light (≥350 nm) and direct sunlight irradiation. In the present work, (Z)-11-hexadecen-1-ol (sex pheromone of Chilo infuscatellus Snellen) was chemically caged by four different photoremovable protecting groups (7-hydroxy-4-hydroxymethylcoumarin, 1-pyrenemethanol, 9-...
متن کاملIdentification of Candidate Olfactory Genes in Chilo suppressalis by Antennal Transcriptome Analysis
Antennal olfaction, which is extremely important for insect survival, mediates key behaviors such as host preference, mate choice, and oviposition site selection. In insects, odor detection is mediated by multiple proteins in the antenna, especially the odorant receptors (ORs) and ionotropic receptors (IRs), which ensure the specificity of the olfactory sensory neuron responses. In this study, ...
متن کاملImplications of an unintended area-wide IPM for Chilo suppressalis in Japan
This paper discusses the success of an unintended area-wide integrated pest management (IPM) for Chilo suppressalis in Japan. Chilo was considered as one of the country’s major rice insect pests causing significant yield loss during the pre-war period, along with Scirpophaga incertulas . Chilo population density declined through the years, which was considered to be induced by modern farming te...
متن کامل